Dynamic quantitative phase imaging for biological objects using a pixelated phase mask

Dynamic quantitative phase imaging for biological objects using a pixelated phase mask

This paper describes research in developing a dynamic quantitative phase imaging microscope providing instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns derived using the polarization properties of light to track dynamic motions and mor...

Pixelated Phase-Mask Dynamic Interferometer

We demonstrate a new type of spatial phase-shifting, dynamic interferometer that can acquire phase-shifted interferograms in a single camera frame. The interferometer is constructed with a pixelated phase-mask aligned to a detector array. The phase-mask encodes a high-frequency spatial interference pattern on two collinear and orthogonally polarized reference and test beams. The phase-differenc...

Quantitative Phase Imaging using Multi-wavelength Optical Phase Unwrapping

4D STEM: High efficiency phase contrast imaging using a fast pixelated detector

Phase contrast imaging is widely used for imaging beam sensitive and weak phase objects in electron microscopy. In this work we demonstrate the achievement of high efficient phase contrast imaging in STEM using the pnCCD, a fast direct electron pixelated detector, which records the diffraction patterns at every probe position with a speed of 1000 to 4000 frames per second, forming a 4D STEM dat...

Computational All-in-Focus Imaging using an Optical Phase Mask

A method for extended depth of field imaging based on image acquisition through a thin binary phase plate followed by fast automatic computational post-processing is presented. By placing a wavelength dependent optical mask inside the pupil of a conventional camera lens, one acquires a unique response for each of the three main color channels, which adds valuable information that allows blind r...